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GLP-1 evoked calcium response in Glp1r + Ghsr + neurons and ghrelin 10 min after GLP-1 did not affect the response (Fig

Functional and phenotypic analyses confirm the induction of a pancreatic endocrine fate, including positive dithizone (DTZ) staining indicative of insulin production, as well as the upregulation of key pancreatic markers such as paired box gene 4 (PAX4), neurogenin 3 (NGN3), Isl-1, Some protocols utilized additional strategies like fibrin encapsulation, decellularized amniotic membrane [52] or 3D alginate encapsulation [51] scaffolds to enhance differentiation efficiency and mimic physiological conditions more closely

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