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The limit of detection for GSH was 0.2 M
In addition, this would not explain the disparity in the effect of exendin 9-39 infusion in people with and without type 2 diabetes, at least in the fasting state, nor the other evidence we previously discussed for islet production of GLP-1
doi:10.1001/jama.2021.23619
The redox effects of GSH or other thiol agents can be assessed by the use of irreversible thiol alkylating agents such as N-ethyl maleimide (NEM) which would form covalent bonds with cysteine residues and prevent further modification of these sites as has been analyzed in modulation of GABA- and glycine-evoked currents in rat retinal ganglion cells (24)
Long-term storage at -20 C maintains stability for 2-3 years
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