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The cells were treated with Hanks Balanced Salt Solution (Hyclone) or 1 mM DTT for 10 min before incubation in Hanks Balanced Salt Solution with different concentrations of GLP-1 (02 M) at room temperature for 20 min

Researchers initially examined it in the context of obesity and metabolic-syndrome research models, exploring whether modulating NNMT alters markers of substrate utilisation and energy expenditure

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