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The green fluorescent protein (GFP) signal was observed using laser confocal microscopy (Leica DMi8, Wetzlar, Germany) in 2 d after injection, with an excitation wavelength of 488 nm and an emission wavelength of 507 nm
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The samples were then sonicated in ice-cold water using Biorupter UCD-200 sonicator for 5 min (30 s sonication and 30 s rest cycle
2017;17:58594.e4
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