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Following overnight selection, plasmids were collected from 35 ml of culture by midiprep following the manufacturers protocols and prepped for high-throughput amplicon sequencing
The results of sensitivity analyses in which outliers were excluded from the data set demonstrated that the observed changes in weight ( p = 0.001), BMI ( p = 0.002) and waist circumference ( p = 0.001) remained statistically significant for both groups
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