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The effect has been replicated within the original research group and is consistent across multiple in vitro reports
Kraft, V
Identification of the exact mechanism for the lethal effect of silencing the enzyme requires further experiments, as do the effects of inhibitors, which are not necessarily solely due to loss of enzyme activity, because the inhibited enzyme may also have a gain of function leading to cell death [72]
The simplicity is intentional: the fewer inactive ingredients in the diluent, the lower the risk of interaction with whatever medication is being reconstituted
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