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10.1186/s40780-017-0083-x 88 SchmidtR

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129,130 It is possible that increases in circulating levels of UA reflect a protective response against oxidative stress that is linked to obesity-associated metabolic syndrome

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Briefly, the sections were deparaffinized in xylene, rehydrated in a graded alcohol series, incubated in 3% hydrogen peroxide to block endogenous peroxidase activity, heated in antigen retrieval solution, subjected to FBS to block nonspecific binding, and incubated with GPX4 antibody (Abcam, ab125066, 1:100) at 4 C overnight