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By RNA seq of cultured mouse hippocampal NSCs electroporated with a REST shRNA vector to knockdown REST , compared with a control empty shRNA vector, authors found a gene expression profile indicative of increased differentiation in REST knockout progenitor cells

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Animal studies indicate dose-dependent effects, with toxic doses possibly affecting copper metabolism or interacting with embryonic development in hypersensitive models, warranting caution during pregnancy

TM3 cells were seeded in 96-well plates at a density of 510^3 cells/well

Refrigeration at 2 to 8 degrees Celsius maintains stability