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Quantitative PCR (qPCR) was performed using FastStart Universal SYBR Green Master (ROX) (Roche) on the Roche LightCycler 480 II Real-Time System

A sensible experiment spreads doses across at least three orders of magnitude, something like 1, 10, and 100 nanomolar on up to 1 and 10 micromolar, precisely because the sweet spot moves with the copper-to-peptide ratio, the buffer, and how much serum sits in the media

MMP-9 (gelatinase B) is involved in degrading damaged basement membrane and facilitating the migration of repair cells (both keratinocytes and fibroblasts) into the wound site

PLoS ONE 9(3):e92214

Patients often say that hair feels stronger or denser after months of treatment