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B12 is administered during an in-office visit, and is injected into muscle (intramuscularly)
But topical application has inherent limitations
Rapid injection can cause localized discomfort and irregular absorption
Sigma, Saint Louis, Missouri) Oxidative stress was assessed by the quantification of protein carbonyls and MDA using Protein Carbonyl Colorimetric and TBARS Assay Kits, respectively (Cayman Chemical, Ann Arbor, MI)
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