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36687-82-8 Added to RFQ For Research & Analytical Purposes
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For the in vitro ubiquitination assay, 0.01 g E1, 0.03 g E2, 0.03 g E3, 0.1 g Pelo, and 0.1 g ubiquitin (ThermoFisher Scientific) were incubated in ubiquitination buffer (1 M Tris, pH 7.2, 100 mM ATP, 200 mM MgCl 2 , 40 mM DTT, 20 ) at 30 C
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