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The cell extracts were added to a series of tubes with/without a constant amount of recombinant R1 and R2 and incubated with the [ 3 H]CDP buffer (100 mM HEPES, pH 7.6, 15 mM magnesium acetate, 10 mM ATP, 200 M CDP, 5 mM DTT, 0.5 mM [ 3 H]CDP (Vitrax, Placentia CA USA, 5548 cpm/nmol) for 30 min at 37 C in a final volume of 90 l

ScarpelliniEValenzaVGabrielliMLauritanoECPerottiGMerraGet al

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