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For recycling, cells were incubated with agonists at 37 C for 15 min, followed by endocytosis arrest at 4 C, surface label removal as above, 30 min incubation at 37 C with 10 M exendin(9-39) to prevent further internalization, and a second round of surface label removal at 4 C

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These vary from person to person and may include nausea, vomiting, fatigue, constipation, and headaches

In general, VPA-poisoned patients are managed with supportive care alone and gastrointestinal decontamination, if appropriate [6]

Application of encapsulation strategies for probiotics: from individual loading to co-encapsulation