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Folate content in plants may be increased by stimulation of folate biosynthesis
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1 mM EGTA, protease inhibitors) containing the respective concentration of digitonin, followed by centrifugation for 10 min at 12,000 g
Briefly, cultures (30 mL) were centrifuged at 8000 g for 10 min at 25 C, and the supernatant was adjusted to pH 6.0 with 2.0 M HCl
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