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Compared with the parental cells, the first prescission cleavage eluates after GFP immunoprecipitation, and final eluates after His-tag affinity chromatography of extracts from cells expressing the tagged wild-type human FUS/TLS contained at least 11 and 17, respectively, additional polypeptides that were observable directly with silver staining (marked with dashed lines, Fig
At least 4 biological replicates were examined
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Magnesium works through osmotic mechanisms
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