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Tissue lysate preparation The ipsilateral coronal brain tissue weighed between 75 and 100 mg, and it was homogenized in chilled condition using a micropestle with 1 ml of either extraction buffer (used for nitrite and malondialdehyde quantification) or radioimmunoprecipitation assay (RIPA) solution containing protease inhibitor
10.1124/jpet.105.093286 27 MaioneS.RadanovaL.De GregorioD.LuongoL.De PetrocellisL.Di MarzoV.et al (2015)
Valproic acid and thalidomide induce morphological abnormalities in the brain, such as reducing cranial motor neurons and modifying cerebellar structures [214, 215]
See our [NAD+ dosing guide](/guides/nad-plus-dosing-guide-how-much-to-take) for protocols, or [explore NAD+ products](/products/nad-001)
