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Thus, it appeared that the introduced disulfide bond would be impossible to analyze with the GSH redox couple (E = -240 mV (Aslund et al., 1997)) as even the best commercial preparations of GSH contain a significant contamination of GSSG (Winther and Thorpe, 2014)
Mendicino M, Bailey Alexander M, Wonnacott K, Puri Raj K, Bauer Steven R
FEMS Microbiol Lett 297:217224 Ishii J, Izawa K, Matsumura S, Wakamura K, Tanino T, Tanaka T, Ogino C, Fukuda H, Kondo A (2009) A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast