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Description

Keep remaining powder frozen for later

Saline is made up of both water and salt

The heterologous expression of the five mutants Phe260Gly, Asn240Lys, Leu244Arg, Asn240Lys/Leu244Arg and Asn240Thr/Leu244Arg yielded adequate amounts of soluble enzyme

On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

SLC27A2, expressed in myeloid cells, is upregulated in sepsis, driving excessive fatty acid influx, mitochondrial dysfunction and metabolic stress