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Summary: A hydroxocobalamin B12 injection typically maintains therapeutic levels for approximately 8 to 12 weeks in most patients, which is why maintenance injections are usually given every 2 to 3 months

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The 20 L reactions contained 12.5 L of TB Green Premix Ex Taq II (Tli RNaseH Plus), 0.4 L of ROX Reference Dye II, 200 ng of cDNA template, 0.4 L of each primer (10 M), and 6.26 L of PCR-grade water