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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26
10.1006/bbrc.2001.5544 Biochem
This supports balanced hormone levels and reduces the risk of side effects often associated with synthetic hormone therapies
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