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To calibrate the probe, fully oxidized and fully reduced samples were generated by the addition of 20 mM N , N , N , N -Tetramethylazodicarboxamide (diamide, Sigma Aldrich ) or 100 mM Dithiotreitol (DTT, AppliChem ), respectively

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Lipodystrophy manifests as abnormal fat distribution, marked by diminished fat in the face and limbs and increased abdominal and visceral fat, often as a side effect of antiretroviral medication

77,78,79 For example, Golodirsen, an ASO drug targeting the human Duchenne muscular dystrophy (DMD) gene, was introduced in the USA in 2019 by Sarepta Therapeutics to treat DMD patients with confirmed mutations amenable to exon 53 skipping

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