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doi: 10.1248/bpb.b14-00780
Because GHK-Cu arrives as lyophilized powder reconstituted to a researcher-chosen concentration, the same target dose corresponds to different syringe volumes depending on the reconstitution choice
So, you would have already purified proteins corresponding to the full-length and each individual domain of the interacting protein, and now you do a pull-down to see which ones bind
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