Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures
Plant Cell 25:45804595 Peltzer D, Dreyer E, Polle A (2002) Differential temperature dependencies of antioxidative enzymes in two contrasting species: Fagus sylvatica and Coleus blumei
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Available Options Single Vial 10ML $7 3 Pack 10ML Each $21 10 Pack 10ML Each $85 Product Features Sterile and non-pyrogenic preparation Contains 0.9% benzyl alcohol Multi-use vial design Commonly used for peptide reconstitution Securely sealed 10ML bottle Suitable for laboratory and research applications Storage Instructions Store in a cool, dry place away from direct sunlight and excessive heat
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