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44 With C-terminal curtailing, the insulinotropic activity of exenatide gradually declines
In brief, an aliquot (200 mg) of each sample was suspended, while frozen, in a solution containing 500 l of freshly prepared extraction buffer (200 mM Tris, 200 mM NaCl, 20 mM EDTA, pH of 8.0), 200 l of 20% SDS and 500 l of a mixture of phenol:chloroform:isoamyl alcohol (25:24:1) in 2 ml VK05 Precellys tubes containing 0.5 mm glass beads (Bertin Technologies)
A large polycarbonate box was used to conduct this test, which was divided into three compartments by two movable walls
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