On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
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The goal is sustainable habit change, which is the entire premise behind the lifestyle interventions studied in major clinical trials like the STEP 1 trial for semaglutide