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This kind of trial is expensive typically several million dollars and to date has been more difficult to justify economically because GHK-Cu cannot be patented as a novel drug (the molecule is endogenous and the relevant patents have expired)
The main areas TB-500 is being studied for and the study-reported figures behind them
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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
Route: The injection is typically given intramuscularly (IM) into a large muscle, such as the thigh or buttock, or subcutaneously (SC) into the subcutaneous tissue
