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After centrifugation at 30,000 g for 30 min, the supernatant fraction was loaded onto a nickelimmobilized metal ion affinity chromatography (NiIMAC) column (His GraviTrap, GE Healthcare)
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E13 (Other specified DM): Includes post-pancreatectomy, postprocedural, monogenic (MODY), and secondary DM NEC
Clinical studies show significant improvements in hair density when used consistently
The assay buffer was composed of 2.5 mM MgCl 2 , 1.0 mM CaCl 2 , 0.003% w/v Tween 20, 0.1% w/v bacitracin (USB corporation) in 25 mM HEPES pH 7.4
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