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Due to the existence of the conjugated system of C = C bonds and carbonyl groups, 4-HNE can provide partial positive charge to the carbon at position three and become efficient electrophiles, allowing it to react with essential biological molecules, including proteins and DNA (107109)
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This suggests the peptide triggers a broad network of healing signals rather than relying on just a single pathway
But only a maximum of 5mcg of B12 enters the bile every day (according to "Advanced Haematology" 7th edition textbook, page 60), so it seems that only an additional 5mcg would be lost daily if none was recovered
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