One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)
Enzymatic properties and physiological roles As already mentioned, owing to the presence of a catalytic cysteine residue, Cys-GSTs have particular enzymatic properties since they should in principle catalyze deglutathionylation reactions by performing nucleophilic attacks on various GSH-conjugated substrates (Board et al., 2000
Transwell assay Treated fibroblasts were placed in the upper chambers of a 24-well transwell plate with 8 m pores (Corning, NY, USA)
AOD 9604 5MG should be stored in a cool, dry environment away from direct light
Methods Hsa-let-7c target genes prediction Prediction and analysis of target genes of hsa-let-7c were performed using bioinformatics methods