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In zebrafish, the colonization of the gut by microorganisms promotes epithelial absorption of fatty acids (Semova et al., 2012) and fish with intact microbiota have increased lipid accumulation in the intestinal epithelium, and increased expression of genes related to lipid metabolism compared to germ-free fish who lack microbiota (Sheng et al., 2018)

Reconstitution quality, sterile technique, and concentration control directly affect whether the intended dose is the dose that gets administered

Head-to-head comparative trials are needed for definitive conclusions

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