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IVF Semen was collected from a single bull, thawed at 37 C for 20 s, and centrifuged at 600xg for 5 min (repated twice) to separate spermatozoa from plasma
The human proteome (FASTA file UP000005640) was obtained from the Uniprot database /uniprot/current release/knowledgebase/referenceproteomes/Eukaryota/UP000005640/), comprising 96,418 protein entries
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