For complete thymosin beta-4, intravenous single doses of up to 1260 mg were well tolerated in healthy subjects, with no dose-limiting toxicity Ruff et al., 2010, and recombinant thymosin beta-4 showed no accumulation Wang et al., 2021
For the E12.5 data, sample-specific relative quality weights were estimated using arrayWeights (Ritchie et al, 2006), and three surrogate variables computed by limmas wsva function were included to account for hidden confounders
Shalansky SJ, Naumann TL, Englander FA
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Immunostaining of brain tissue for Iba1 and GFAP revealed that microglia and astrocytes were markedly activated in GCLC-KO mouse brains (Fig