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Muscles were evaluated for nitration levels of ECM-bound HGF by direct-immunofluorescence microscopy with HiLyte Fluor 647-labeled anti-nitrated Y198-HGF mAb (clone 3A11C6, fluorescent false-yellow) in cross-sections of specimens (Figure 2e)
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Aerobic exercise protocol Rats in the DE and DME groups were trained on a treadmill exercise protocol based on the Bedford motion model protocol 41 , using a speed of 15 m/min for 3 days for acclimatization, after which the treadmill speed was increased to 20 m/min for 60 min/day
