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Similarly, transgenic male C57BL/6J mice expressing an activated rat c-neu oncogene (Erbb2) under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) promoter (MMTV-NeuT/Erbb2 77 ), were crossed with the Ubiad1 +/ females to generate MMTV-NeuT
doi:10.1097/ede.0000000000000804
Proteins were transferred onto a NC or PVDF membrane (Fisher Scientific) and blocked with 5% milk for 1 h
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