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If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
Unlike other compounds that work alone to neutralize oxidative stress, glutathione has a unique role in recycling and sustaining the activity of antioxidants such as vitamins C and E
Protocol design is based on skin tone, pigmentation severity, treatment goals, overall health profile, and any concurrent medications or conditions
(Wang et al., 2022)
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