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The gap detection test measures the animals ability to detect silent intervals within continuous pure tones, while PPI assesses the animals response to pure tone pulses presented during silent intervals

Inhibition of GPR43/GPR109a expression by RNA interference Human G-protein-coupled receptor 43 (GPR43) or GPR109a siRNA or negative control (NC) siRNA was mixed with the Lipofectamine 2000 transfection reagent (Invitrogen, California, USA) (Table S1)