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The cDNA was amplified using a Syber Green I PCR-Master Kit from Fermentas (PA, United States)

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In the context of reproduction, the observed impaired spermatogenesis, hormonal imbalances, and reduced litter sizes are direct consequences of aberrant cell division and gene expression in reproductive tissues [85, 97]

and reviewed by Pickart and Margolina found that GHK modulates expression of approximately 31 percent of analyzed human genes, with the net effect resetting age-shifted expression patterns toward younger profiles 9 1

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