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It has been studied specifically in Achilles tendon, rotator cuff, patellar tendon, and other tendon injury models

Risk of liver injury among methotrexate users: a meta-analysis of randomised controlled trials

A reasonable workup includes basic laboratory testing, infectious serologies (hepatitis B and C, human immunodeficiency virus), serum protein electrophoresis, direct immunofluorescence studies with immunoglobulins (IgG, IgA, IgM), antinuclear antibodies, rheumatoid factor, serum complement levels, antineutrophil cytoplasmic antibodies, and cryoglobulins

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