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Mechanistic studies using CRISPR/Cas9-engineered cell lines bearing the C65G mutation revealed a multilayered disruption of tRNA [Ser]Sec function: reduced tRNA expression, severe impairment of selenoprotein synthesis, and most strikingly a loss of selenium responsiveness not observed with other tRNA [Ser]Sec mutants [111]
We think it is inappropriate for physicians to prescribe a critical drug that is in short supply to treat an unapproved condition
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After screening 258 human GPCRs, we identified GPR55 as a potential target of curcumin, based on a significant activation of the reporter construct by curcumin
However, regulatory guidance states that when the ratio of the area under the concentration-time curve (AUC) of the victim drug with and without the perpetrator drug falls outside the range of 0.81.25, there is a potential risk of a clinically significant impact, necessitating further investigation
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