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Moreover, while many studies emphasize its health-promoting effects, inconsistencies remain regarding bioavailability, optimal dosage, technological compatibility in food matrices, and large-scale production sustainability (16, 17)

Some practitioners also advocate NAD+ therapy for addiction recovery, chronic fatigue, and neurodegenerative conditions, though scientific support for these applications remains insufficient

Cresyl violet staining Brains were harvested after the mice were perfused transcardially using icecold PBS solution

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