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We propose that NADPH and GSSG convey TCA cycle status: decreasing the availability of acetyl-CoAeither from lipid or carbohydrate catabolismdampens NADPH production and disfavors glutathione reduction, with the increased GSSG ultimately rendering the ER lumen more oxidizing and protecting ER homeostasis

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We observed a change in the fluorescence lifetime from 1.3 ns in an NADP + -saturated state to 2.3 ns in an NADPH-saturated state, which is exceptionally large for a fluorescent protein-based biosensor

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