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This two-step binding process, where the C-terminal region of the peptide ligand binds to the N-terminal domain of the receptor, allowing the N-terminal region of the peptide ligand to interact with the helical region of the receptor and resulting in receptor activation, has since been confirmed by numerous structures determined by X-ray crystallography and cryo-EM (Underwood et al., 2010
Yes, but temperature management is critical
To visualize the biodistribution of nanoparticles in mice, NP4 was labeled with the fluorescence dye Cy7.5, resulting in NP4-Cy7.5
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