Western blot Cells were washed with PBS and lysed in cell lysis buffer (50 mM Tris, pH 7.4, 1% Triton, 150 mM NaCl, 10% glycerol, supplemented with protease and phosphatase inhibitors)
Its a structured dose escalation ranging from 2.5 mg to 15 mg
I tried to do a short workout at my apartment gym and planned for twenty minutes on the StairMaster, but my body gave up after two minutes
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J Clin Invest 112:18311842 Zhang CY, Baffy G, Perret P et al (2001) Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, beta cell dysfunction, and type 2 diabetes