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To explore the functional role of FOXO4 in senescence, its expression was silenced using siRNA in TM3 Leydig cells prior to H2O2-induced senescence

Patients should be encouraged to: Consume a balanced diet rich in calcium (dairy products, fortified plant-based alternatives, leafy greens) Ensure adequate protein intake to preserve lean muscle mass during weight loss Engage in regular physical activity following UK Chief Medical Officers' guidelines Limit alcohol consumption to no more than 14 units per week, spread over 3 or more days Avoid smoking, which impairs bone health Maintain safe home environments to reduce fall risk When to seek medical advice : Patients should contact their GP or diabetes specialist nurse if they experience unexplained bone pain, sustain a fracture from minimal trauma, or have concerns about their bone health

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