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In another investigation, a model was employed wherein gelatin methacryloyl hydrogel microchannels were either solely covered by HUVECs without fibroblasts (serving as the control), devoid of endothelial lining but containing encapsulated fibroblasts within the hydrogel, or with both endothelial lining along with encapsulated fibroblasts embedded within the matrix

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If your protocol calls for very small doses (under 50 mcg), consider 2 mL instead to make measurements more practical