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(2) chiral / stereochemistry confirmation (chiral HPLC or amino acid analysis) because the two critical D-amino acid residues (D-2-Nal, D-Phe) cannot be distinguished from L-isomers by standard reverse-phase HPLC

Therefore, the use of several sub-strains of mice is a strength of this study [49, 50], and suggests that our findings are more likely to generalize to other mouse sub-strains than they would have if the study were conducted in only one strain of mice

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& Steinberg, W

Findings remain preliminary, and further investigation is required to clarify their roles