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Diagnosis typically involves blood tests including full blood count (showing macrocytosis), anti-intrinsic factor antibodies (which are specific for pernicious anaemia), and sometimes anti-parietal cell antibodies

The compound's mechanism of action involves interaction with specific receptors, which can lead to both intended effects and unintended consequences that researchers classify as side effects

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Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies