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Vitamin B 12 is stored as active coenzyme, with a turnover rate of 0.5 to 0.8 mcg per day depending on the body shell capacity

The secondary antibody used was the BrightVision poly HRP-Anti-Rabbit IgG, incubated for 45 min (DPVR-110HRP, ImmunoLogic) or the polyclonal goat Anti-Mouse at a dilution of 1:100 for 30 min (Dako-Agilent, P0447)

The intact sucrosome vesicles facilitate absorption through both paracellular and transcellular routes, potentially involving uptake by enterocytes and M cells (2729, 37)

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salvage pathway