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This response is independent of driver oncogene and environmental conditions but specific to cancer cells, both in vitro and in vivo, further highlighting the inherent mitochondrial plasticity in cancer
Glutathione, or glutamyl-cysteinylglycine (GSH), is the primary intracellular antioxidant involved in preventing lipid and protein oxidation and is a critical component in the enzymatic process used to detoxify hydroxide peroxide (H2O2)
Function, Oxidative, and Inflammatory Stress Parameters in Immune Cells as Predictive Markers of Lifespan throughout Aging
After 24 h transfection, cells were resuspended with ice-cold GST-lysis buffer (20 mM Tris-HCl, pH 8.0, 1% Triton X-100 and 10% glycerol in PBS) supplemented with a protease inhibitor cocktail (Roche) and 50 M Palmostatin B (for SwissKASH-derived samples), and passed 10 times through a 25 G needle
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