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PCR products were isolated and cloned for DNA sequencing using a Zero Blunt TOPO PCR Cloning Kit and chemically competent TOP10 E.coli cells (Life Technologies)

This multi-pathway mechanism supports appetite regulation, glucose metabolism, and energy expendituremaking it a focus of research in obesity, metabolic disease, and endocrinology

Feb 10, 2026 One wrong measurement and your entire vial is compromised